Kinetics of Reductive Dechlorination of Trichloroethane (TeA) by Anaerobic Biofilms
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Detoxification of 1,1,2-Trichloroethane to Ethene by Desulfitobacterium and Identification of Its Functional Reductase Gene
1,1,2-trichloroethane (1,1,2-TCA) has become a common groundwater pollutant due to historically extensive utilization, improper disposal, as well as from incomplete dechlorination of 1,1,2,2-tetrachloroethane. Currently, limited information is available on microbial detoxification of 1,1,2-TCA. Desulfitobacterium sp. strain PR, which was isolated from an anaerobic bioreactor maintained to dechl...
متن کاملGrowth of Dehalobacter and Dehalococcoides spp. during degradation of chlorinated ethanes.
Mixed anaerobic microbial subcultures enriched from a multilayered aquifer at a former chlorinated solvent disposal facility in West Louisiana were examined to determine the organism(s) involved in the dechlorination of the toxic compounds 1,2-dichloroethane (1,2-DCA) and 1,1,2-trichloroethane (1,1,2-TCA) to ethene. Sequences phylogenetically related to Dehalobacter and Dehalococcoides, two gen...
متن کاملKinetics and inhibition of reductive dechlorination of trichloroethene, cis-1,2-dichloroethene and vinyl chloride in a continuously fed anaerobic biofilm reactor.
Anaerobic bioreactors containing Dehalococcoides spp. can be effective for the treatment of trichloroethene (TCE) contamination. However, reductive dehalogenation of TCE often results in partial conversion to harmless ethene, and significant production of undesired cis-1,2-dichloroethene (cis-DCE) and vinyl chloride (VC) is frequently observed. Here, a detailed modeling study was conducted focu...
متن کاملEffects of transition metal and sulfide on the reductive dechlorination of carbon tetrachloride and 1,1,1-trichloroethane by FeS.
Reductive dechlorination of carbon tetrachloride (CT) and 1,1,1-trichloroethane (1,1,1-TCA) by FeS with transition metals (Cu(II), Co(II), and Ni(II)) and hydrosulfide was characterized in this study. The batch kinetic experiments were conducted by spiking each stock solution of CT and 1,1,1-TCA into 33 g/L of FeS suspensions with and without transition metals at pH 7.5. No significant enhancem...
متن کاملIdentification of Dehalobacter reductive dehalogenases that catalyse dechlorination of chloroform, 1,1,1-trichloroethane and 1,1-dichloroethane.
Two novel reductive dehalogenases (RDases) that are highly similar to each other but catalyse distinct dechlorination reactions were identified from Dehalobacter-containing mixed cultures. These two RDases were partially purified from crude protein extracts of anaerobic dechlorinating enrichment cultures using blue native polyacrylamide gel electrophoresis. Gel slices were assayed for dechlorin...
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تاریخ انتشار 2000